Blood Grouping ABO And Rh Typing, EDTA Whole Blood

The ABO grouping and Rh typing test determines blood type grouping (A, B, AB, O ) and the Rh factor (positive or negative). A person's blood type is based on the presence or absence of certain antigens on the surface of their red blood cells and certain antibodies in the plasma. ABO antigens are poorly expresses at birth, increase gradually in strength and become fully expressed around 1 year of age. Blood grouping and typing is used to determine the compatibility between donar and recipient of blood or its components. Compatibility of blood grouping and typing is very important as incompatible blood transfusions leads to fatal outcome. For example, if recipient blood group is group O, he has both antibodies A and B in his blood, and if he is transfused with A, B or AB blood group, the antibodies present in the recipients blood react with the donars red blood cells which has the antigens, destroy them and that results in serious complications. Similar reactions occur when a Rh positive donar gives blood to a Rh negative recipient, but the complications are not seen mostly during the current transfusion and occur with the future transfusion. Example the Rh typing is important during pregnancy, especially if the mothers blood group is Rh negative and fathers blood group is Rh positive, because in such situations the fetus may have Rh positive blood group and hence the mother can develop antibodies against the fetus red blood cells which contain antigens. These antibodies may cross the placenta and destroy the baby blood cells, resulting in haemolytic disease of new born or fetus. The result of this incompatability may not be seen in the current pregnancy but can effect the future pregnancy. To prevent this, the Rh negative mother is given Rh immunoglobulins during pregnancy and immediately after delivery. These immunoglobulins will mask the fetus Rh antigens and prevent such complications. Blood grouping and typing is also important in donors and recipients of organ or bone marrow transplantation, along with HLA testing.